To study the impacts of environmental regulation on enterprise green innovation and roles/mechanism of artificial intelligence between environmental regulation and enterprise green innovation, this paper, based on 2000 to 2019 data of China's A-listed manufacturers, uses bi-directional fixed effect model to their relation among environmental regulation, artificial intelligence and enterprise green innovation.Environmental regulation can dramatically improve enterprise green innovation, which also can be boosted by applying artificial intelligence through mitigating enterprise financing constraints, increasing whole element productivity and saving laboring costs.Environmental regulation cannot improve the green innovation level of state-owned enterprises, but the application of artificial intelligence can enhance the role of environmental regulation in improving the green innovation level of state-owned enterprises.Environmental regulation can improve the green innovation level of non-state-owned enterprises, and the application of artificial intelligence can enhance the role of environmental regulation in improving the green innovation level of non-state-owned enterprises.Environmental regulation cannot improve the green innovation level of small-scale enterprises, and the application of artificial intelligence cannot enhance the role of environmental regulation in improving the green innovation level of small-scale enterprises.Environmental regulation can improve the green innovation level of large-scale enterprises, and the application of artificial intelligence can enhance the role of environmental regulation in improving the green innovation level of large-scale enterprises.Environmental regulation cannot improve the level of green innovation of labor-intensive enterprises, and the application of artificial intelligence cannot enhance the role of environmental regulation in improving the green innovation level of labor-intensive enterprises.Environmental regulation can improve the green innovation level of capital-intensive enterprises, and the application of artificial intelligence can enhance the role of environmental regulation in improving the green innovation level of capital-intensive enterprises.Environmental regulation cannot improve the green innovation level of technology-intensive enterprises, but the application of artificial intelligence can enhance the role of environmental regulation in improving the green innovation level of technology-intensive enterprises.